Applejack, also known as Princess Applejack, is a tomboyishly beautiful orphaned orange earth pony mare and the pentagonist in My Little Pony: Friendship is Magic. She lives at Sweet Apple Acres with her grandmother Granny Smith, big brother Big Macintosh and adorable and pretty little sister Apple Bloom. She wields lasso as her signature weapon. In My Little Pony: Armed Stallions, she urges Soarin' (Matthew Hill), Thunder Lane (Trevor Devall), and Fancy Pants not to trust both Victor Hoskins and Dr. Henry Wu (B.D. Wong).
In My Little Pony: The Book of Golden Oak, she serves as a secondary supporting character who made her first debut in the season 1 episode 2 "An Undercover Cowgirl", where she is shown working and spying for the Shredder (Kevin Michael Richardson) and his men, but it turns out she isn't. She is working undercover by the Canterlot Intelligence Agency under orders to retrieve data on the Boiling Liquid Expanding Mutant Creation (BLEMC) and kill the Shredder and his men once and for all. Nevertheless, due to occasionally standing against other heroes from non-Hasbro franchise, she is considered by the other heroes, especially Leonardo, Michelangelo, Donatello, Raphael, Casey Jones, and April O'Neil as a foremost adversary/rival. In My Little Pony: One Day at Jurassic World, Applejack appears in Dr. Henry Wu's prologue and serves as the main narrator of events while spying on the treacherous Chinese American mad scientist.